BioWizard » Thu Mar 16, 2017 6:46 pm wrote:Probably both. Getting DNA from nice live cells is going to be better (less fragmented) than getting it from dead/dying keratinized cells. And extraction technique will affect purity and yeild.
This is what New England Biolabs recommend at their website:
For low complexity templates (i.e. plasmid, lambda, BAC DNA), use 1 pg–10 ng of DNA per 50 µl reaction
For higher complexity templates (i.e. genomic DNA), use 1 ng–1 µg of DNA per 50 µl reaction
You can see the range is quite broad. Usually for small genomes you would use lower concentrations, since you expect more copies of your target sequence per ng.
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